GAMBOA, L.; DÍAZ, M.; GÓMEZ, J.; SEGURA, C.; THOMET, F; TESSINI, C.:
COLACRO 2019, Abstract Book (157).
Abstract
The objective of this work is to perform the solid phase extraction of the catechol present in a fraction of the bio-oil and perform the breakthrough curves on a C-18 cartridge. The bio-oil, contains water, sugars, acids and phenols depending on the biomass used. Catechol is used for the manufacture of pesticides, in the cosmetic and pharmaceutical industry. Currently this compound has great interest as an electrolyte in redox flow batteries, due to its electroactive properties. According to this, the scientific efforts are focused on looking for organic electroactive compounds but that are obtained from renewable sources since one of the organic compounds that is currently used for the operation of these is the catechol obtained in a synthetic form. For this reason, the extraction of this compound is of great interest since it contributes to the development of renewable energies. In this work, an analytical method was validated by HPLC-DAD, using a C18 250×4.6 mm x 5 μm column, with an acetonitrile/phosphate buffer pH = 2.5 gradient, obtaining 8.69 ± 0.02% catechol and 3.37 ± 0.02% of 4-methylcatechol, as major compounds. In the second stage, an extraction of these compounds was carried out by SPE and the breakthrough curves were made using standards and bio-oil. For this, the sample volume was varied at a constant concentration. The range of this volume was between 0.5 to 10 mL, finding that the breakthrough volume of the catechol considering 1% of C/Co was 3 mL. The determination of the maximum capacity was made by varying the concentration of the standards to a constant volume. According to this methodology, it was possible to separate the catechol with a higher performance and purity in the first elution with water and the 4-methylcatechol was eluted with methanol.